The American Red Cross of Yolo County has been present in this area since the late 1800s. The chapter has been located since 1953 at its present site, 120 Court Street, Woodland. The current building underwent an extensive expansion and renovation in 1998 due to several major bequests left to the chapter by long-time volunteers. They included Loraine Huston Chalmers, who made the lead gift for the renovation project; Lillian O. White; and the Mr. & Mrs. G. Kirk Swingle Foundation. Memorials were given for the building project by William Bryan Mitchell, Jr., Bernie Gorman, Ann Kinsman, Edith Swafford and Dr. Frank Summers.

The chapter is located in the Central Valley, about 20 miles northeast of Sacramento and 75 miles east of San Francisco. Its major cities include Davis, which houses the University of California at Davis, Woodland, the county seat, and Winters.